E10187
Invitrogen™ EdU (5-ethynyl-2'-deoxyuridine)
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | E10187-Each-of-1 | In Stock | ₹ 1,60,168.32 |
E10187 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,60,168.32
GST (18%): ₹ 28,830.298
Total Price: ₹ 1,88,998.618
Detection Method
Fluorescence
Product Type
EdU
Shipping Condition
Room Temperature
Label or Dye
EdU
Product Line
Molecular Probes™, Click-iT™
Format
Solid
Content And Storage
Store at ≤-20°C.
Sample Type
Any Tissue Type, Cell Cultures, Tissue Sections, Cultured Cells, Whole Animals
Quantity
500 mg
Related Products
Description
- EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry
- Click chemistry is a method of covalently coupling an azide with an alkyne
- Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring
- Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA
- Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS)
- EdU is readily soluble in DMSO, alcohol, water, or aqueous buffers
- For use with in vitro applications, prepare 10 mM EdU stock solution in DMSO or aqueous buffer
- For Research Use Only
- Not intended for any animal or human therapeutic or diagnostic use
- Order Info Shipping Condition: Room Temperature