12-351-250

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 12-351-250-Each-of-1 In Stock ₹ 4,44,912.00

12-351-250 - Each of 1

₹ 4,44,912.00

In Stock

Quantity

1

Base Price: ₹ 4,44,912.00

GST (18%): ₹ 80,084.16

Total Price: ₹ 5,24,996.16

Content And Storage

5 boxes of Platinum SuperFi Master Mix, each containing:• Platinum SuperFi DNA Polymerase (250 μL at 2 U/μL)• 5X SuperFi Buffer (6 x 1.25 mL)• 5X SuperFi GC Enhancer (4 x 1.25 mL)Store at –5°C to –30°C.

Polymerase

Platinum SuperFi DNA Polymerase

Shipping Condition

Wet or Dry Ice

Fidelity (vs. Taq)

300X

No. of Reactions

5 x 500 Reactions

Reaction Format

Separate Components

GC-Rich PCR Performance

High

Reaction Speed

Fast

For Use With (Application)

Hot-start PCR, High-fidelity PCR

Hot Start

Built-In Hot Start

Overhang

Blunt

Size (Final Product)

20 kb or less

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Description

  • Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR
  • Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy
  • Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors
  • This feature also enables fast-cycling protocols and amplification of long targets
  • The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation
  • Benefits of Platinum SuperFi DNA Polymerase Exceptional >300X Taq fidelity High specificity and increased yields with Platinum hot-start technology Robust amplification of difficult targets including those of sub-optimal purity or high GC content Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions Amplification of samples with suboptimal purity Applications High-fidelity PCR Cloning and sub-cloning Site-directed mutagenesis Amplification of GC-rich templates Template generation for sequencing High-throughput PCR Long PCR Notes Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).