12-595-100
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-595-100-Each-of-1 | In Stock | ₹ 99,934.08 |
12-595-100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 99,934.08
GST (18%): ₹ 17,988.134
Total Price: ₹ 1,17,922.214
Content And Storage
• SuperScript IV RT Mix (50 μL)• 2X Platinum SuperFi RT-PCR Master Mix (2 x 1.25 mL)• ezDNase Enzyme (100 μL)• 10X ezDNase Buffer (100 μL)• Nuclease-free water (2 x 1.25 mL)Store at –20°C.
Format
Kit
PCR Method
1-step RT-PCR
Reaction Speed
10 min.
Optimal Reaction Temperature
45°C to 60°C
Ribonuclease H Activity
Reduced
Final Product Type
PCR Amplified cDNA
No. of Reactions
100 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Detection Method
Gel Electrophoresis
GC-Rich PCR Performance
High
Polymerase
Platinum SuperFi
Technique
1-step RT-PCR
Reverse Transcriptase
SuperScript IV
Shipping Condition
Dry Ice
Hot Start
Built-In Hot Start
Reaction Format
Master Mix
Size (Final Product)
Up to 14 kb
Includes
Kit with ezDNase
Related Products
Description
- Description The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance
- With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube
- Features of the SuperScript IV One-Step RT-PCR System include: • Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol • Reliable target detection even with suboptimally pure RNA samples • Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup • Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only) SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis
- Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR
- The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits
- The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.