18-010-025
Invitrogen™ DNA Polymerase I
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-010-025-Each-of-1 | In Stock | ₹ 47,058.00 |
18-010-025 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,058.00
GST (18%): ₹ 8,470.44
Total Price: ₹ 55,528.44
Content And Storage
1000 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μl.Store at -5°C to -30°C.
Polymerase
DNA Polymerase I
Quantity
1000 U
Hot Start
No
Description
DNA Polymerase
Exonuclease Activity
3' - 5', 5' - 3'
Shipping Condition
Wet Ice
Related Products
Description
- Performance and quality testing Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined
- Unit definition One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer
- Unit reaction conditions 50mM potassium phosphate (pH 7.0), 6.7mM MgCl 2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [ 3H ]dTTP, and enzyme in 100μL for 30 min at 37°C
- Also incorporates biotinylated nucleotides
- Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs Source: purified from E
- coli expressing the DNA Polymerase I gene on a plasmid Cloning, Restriction Enzyme Cloning Order Info Shipping Condition: Wet Ice