Thermo Scientific™ Phusion™ Plus DNA Polymerase PROMO

Description

• Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing
• It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance
• High fidelity–provides >100X higher sequence accuracy than Taq polymerase Convenient setup–enables a universal annealing temperature of 60°C in PCR Enhanced specificity–hot-start mechanism reduces non-specific amplification and primer degradation Benchtop stability–automation enabled due to 24-hour stability of assembled reactions High inhibitor tolerance–works with DNA of suboptimal purity The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C
• This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs
• Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol
• Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature
• Enzyme activity is fully restored after the initial denaturation step
• Applications High-fidelity PCR Cloning Template generation for sequencing GC-rich PCR Long-range PCR (up to 20 kb) Mutagenesis High-throughput PCR To simplify PCR reaction setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats
• Phusion Plus PCR Master Mix (Cat
• No
• F631S) is a convenient 2X mix of enzyme, reaction buffer, magnesium chloride, and dNTPs
• Only template and primers need to be added to the PCR reaction
• Phusion Plus Green PCR Master Mix (F632S) additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels.