Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents

Description

• The Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells
• Pre-designed primers and TaqMan™ probe eliminate assay design Rapid assay development guidelines can minimize optimization time Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation
• These guidelines are simple and easy to follow
• Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time
• Normalization Built-In All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations
• Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation
• The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube
• Notes on Use: The TaqMan™ Rodent GAPDH Control Reagents can be used with TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR)
• The TaqMan™ Rodent GAPDH Control Reagents can be used with TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR)
• Note: See user's manual or package insert for limited label license, and trademark information
• For Research Use Only
• Not for use in diagnostics procedures.