Applied Biosystems™ SYBR™ Green Universal Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-127-04-Each-of-1 | In Stock | ₹ 4,13,254.80 |
43-127-04 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,13,254.80
GST (18%): ₹ 74,385.864
Total Price: ₹ 4,87,640.664
Content And Storage
The 10 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 2000 reactions based on a 50 μl reaction volume.Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format
Tube
PCR Method
qPCR
Reaction Speed
Standard
Label or Dye
SYBR Green
Product Line
SYBR™
Quantity
10 x 5 mL
For Use With (Application)
Gene Expression
No. of Reactions
2000 Reactions
Volume
10 x 5 mL
Detection Method
SYBR
GC-Rich PCR Performance
High
Polymerase
AmpliTaq Gold DNA Polymerase
For Use With (Equipment)
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye
ROX (Pre-mixed)
Product Type
Real Time PCR SYBR Master Mix
Sufficient For
2000 Reactions of 50 μL
Concentration
2X
Sample Type
DNA (Genomic), cDNA
Related Products
Description
- Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase Proprietary buffer enhancements ensure performance and reliability Premixed components stored at 2° to 8°C significantly reduce assay setup time Maximum Flexibility and Convenience: Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed
- Store at -20°C Order Info Shipping Condition: Wet Ice