Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-873-91-Each-of-1 | In Stock | ₹ 1,68,895.44 |
43-873-91 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,68,895.44
GST (18%): ₹ 30,401.179
Total Price: ₹ 1,99,296.619
Content And Storage
Sufficient for 1000 reactions of 25 μL, contains:• 14 mL 2X RT-PCR Buffer• 1100 μL 25X RT-PCR Enzyme Mix• 25 mL Nuclease-free Water.
Detection Method
Primer-probe
GC-Rich PCR Performance
High
Polymerase
AmpliTaq Gold DNA Polymerase
For Use With (Equipment)
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line
AgPath-ID™, Ambion™
Reverse Transcriptase
ArrayScript™
Sufficient For
1,000 Reactions
Fidelity (vs. Taq)
25 X
Sample Type
RNA
Description
Works on all AB platforms, but is optimized for the 7500.
Format
Tube
PCR Method
1-step RT-qPCR
Reaction Speed
Standard
Passive Reference Dye
ROX (Pre-mixed)
Product Type
One-Step RT-PCR Reagent
Shipping Condition
Dry Ice
Concentration
25X
No. of Reactions
1000 Reactions
Volume
14 mL
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Description
- Recommended for RNA pathogen amplification Consistent amplification of RNA targets with high specificity and sensitivity Optimized to work with your target-specific primers and probes Contains ROX for quantitative fluorescent signal normalization AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup
- The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time
- Once assembled, results are available in approximately one hour
- The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification
- The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization
- A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.