Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Description

• Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates
• The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb
• RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation at temperatures up to 55°C
• The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT) 18 and random hexamer primers
• The oligo(dT) 18 primer anneals selectively to the poly(A) tail of mRNA
• Random hexamer primers do not require the presence of the poly(A) tail; they can therefore be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs)
• Gene-specific primers may also be used with the kit
• Radioactively- and non-radioactively-labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays
• Features of the RevertAid First Strand cDNA Synthesis Kit include: • Full-length first strand cDNA up to 13 kb • Optimum reaction temperature 42°C • Complete kit with all components necessary for the RT reaction already included Applications • First strand cDNA synthesis for RT-PCR and RT-qPCR • Construction of full length cDNA libraries • Antisense RNA synthesis