Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Description

• Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time
• Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols
• The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less)
• The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase
• Features Extreme speed with extension times of 15 s/kb or less Hot start modification allows 'zero-time reactivation' Accurate proofreading DNA polymerase with a fidelity of 25X Taq High yields in reduced cycling time Minimized pipetting steps Applications Fast PCR High-fidelity PCR Difficult (GC-rich) templates Template generation for sequencing Multiplex PCR Long-range PCR Cloning & mutagenesis Microarray Notes Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).