Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Description

• Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects
• The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle)
• The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues
• Features Direct PCR reduces time-consuming and expensive DNA purification steps Available Dilution & Storage protocol allows multiple PCR reactions from a single sample Specially engineered Phire DNA polymerase for extremely short PCR protocol times Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel Applications Genotyping Transgene detection Knockout analysis Sequencing Notes The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.