Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit

Description

• Ambion Fast SYBRGreen Cells-to-C T ™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results
• A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely
• The kit includes sufficient reagents for 100 reactions
• The Fast SYBRGreen Cells-to-C T Kit contains AmpliTaq™ Fast DNA Polymerase, UP, which allows instant hot start, thus minimizing non-specific product formation with fast real-time PCR
• Includes optimized reagents to work efficiently, right out of the box Simple, effective Cells-to-C T technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA 2-Step qRT-PCR Extraordinary Value The kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBRGreen
• Extraordinary Ease The protocol begins with a simple 7-minute sample preparation procedure
• First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; Dnase treatment can be performed concurrently
• The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at -20°C for up to 5 months
• Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility
• Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix
• Extraordinary Performance To demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the Fast SYBRGreen Cells-to-C T Kit
• The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells
• In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits
• Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms including those from Applied Biosystems, Roche, and Bio-Rad
• Accessory Product: The SYBRGreen Cells-to-C T Control Kit (Cat
• No
• 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-C T reactions
• Order Info Shipping Condition: Dry ice