4919380

Invitrogen™TaqDNA Polymerase, native

TaqDNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Manufacturer: Fischer Scientific

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Format

Stand-alone enzyme

Polymerase

TaqDNA Polymerase

Exonuclease Activity

5' - 3'

Shipping Condition

Wet or Dry Ice

Concentration

5 U/μL

Hot Start

No

Reaction Format

Separate Components

Starting Material

DNA

GC-Rich PCR Performance

Low

Reaction Speed

Standard

Product Type

DNA Polymerase

For Use With (Application)

Standard PCR

Fidelity (vs. Taq)

1X

Overhang

3'-A

Size (Final Product)

5 kb or less

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Description

  • Taq DNA Polymerase, native, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer
  • This native enzyme is purified from Thermus aquaticus YT1 and consists of a single polypeptide with a molecular weight of 94 kDa
  • It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity
  • Features A choice of recombinant or native enzyme Amplification of PCR products up to 5 kb in size Enzyme is licensed and qualified for PCR Applications Amplification of DNA from complex genomic, viral, and plasmid templates RT-PCR Sequencing ssDNA Cycle sequencing Notes One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min
  • at 74°C
  • Order Info Shipping Condition: Approved for shipment on wet or dry ice