Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™TaqHigh Fidelity DNA Polymerase

Description

• The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR
• This one-step formulation allows for both cDNA synthesis and PCR amplification to be performed in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA
• It also allows for the detection of a wide range of RNA targets (up to 10 kb in length) at variable concentrations (1 pg to 1 μg of total RNA)
• This system consists of two major components: SuperScript III RT/Platinum Taq High Fidelity enzyme mix and 2X reaction mix
• SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA. Platinum Taq DNA Polymerase High Fidelity is a an enzyme mixture composed of recombinant Taq DNA polymerase, Pyrococcus species GB–D polymerase, and Platinum Taq antibodies, which block polymerase activity at ambient temperatures enabling hot start PCR
• The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg 2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers
• The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity offers: • High-fidelity end-point detection and analysis of RNA molecules by RT-PCR • PCR amplification and cDNA synthesis in a single tube • Detection of a wide range of RNA targets from 300 bp to 10 kb • Increased specificity with wide cDNA synthesis temperature range of 45−60°C Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System (Cat
• No
• 12594025) or SuperScript IV One-Step RT-PCR System with ezDNase (Cat
• No
• 12595025)
• The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples
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