4926558
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
Manufacturer: Fischer Scientific
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Format
Stand-alone Enzyme
Reaction Speed
30 to 50 min.
Optimal Reaction Temperature
50°C
Ribonuclease H Activity
Reduced
Concentration
200 U/μL
Reaction Format
Separate components
Size (Final Product)
Up to 12.3 kb
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reagent Type
Reverse Transcription
Starting Material
RNA
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Description
- Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability
- SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes
- SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E
- coli containing the modified pol gene of Moloney Murine Leukemia Virus
- Features of SuperScript III Reverse Transcriptase include: • First-strand cDNA may be synthesized at temperatures up to 55°C • cDNA can be generated from 100 bp to >12 kb • Increased specificity • High yields of cDNA and full-length products Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase (Cat
- No
- 18090010), features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity
- Order Info Shipping Condition: Dry ice