Applied Biosystems™PowerSYBR™ Green RNA-to-CT™1-StepKit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-591-795-Each-of-1 | In Stock | ₹ 71,870.40 |
50-591-795 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 71,870.40
GST (18%): ₹ 12,936.672
Total Price: ₹ 84,807.072
Content And Storage
Sufficient for 200 reactions at 50 μL reaction volume and includes:• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
PCR Method
1-step RT-qPCR
Reaction Speed
Standard
Label or Dye
SYBR Green
Product Line
RNA-to-CT™, SYBR™
Purity or Quality Grade
UP (Ultra Pure)
Run Time
Standard
Sufficient For
200 Reactions
No. of Reactions
200 Reactions
Detection Method
SYBR
Polymerase
AmpliTaq Gold DNA Polymerase
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Passive Reference Dye
ROX (Pre-mixed)
Product Type
One-Step qRT-PCR Kit
Reverse Transcriptase
ArrayScript™ UP
Shipping Condition
Wet Ice
For Use With (Application)
Gene Expression
Sample Type
RNA
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Description
- The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets
- Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction
- Formulated to ensure maximum sensitivity and reliability Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets Includes: AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals RNase inhibitor, to prevent degradation of RNA templates A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments