Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits

Description

• The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins
• Reliable and rapid, the resDNASEQ system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of human cell DNA
• This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples to final product, whether the sample contains high molecular weight or sheared DNA
• Features of the resDNASEQ Human Residual DNA Quantitation Kit include: • Highly sensitive quantitation using proven TaqMan real-time qPCR technology • Consistent performance across the expected range of DNA fragment sizes • Includes master mix, TaqMan primer/probe mix, and human genomic DNA standard This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
• The resDNASEQ Human Residual DNA Quantitation Kit provides highly sensitive detection of human DNA, allowing you to use small sample volumes to generate accurate results
• The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of human DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts
• Easy to use With the resDNASEQ Human Residual DNA Quantitation Kit you perform qPCR analysis to compare the amount of human DNA in your test samples to a standard curve generated with known amounts of purified human genomic DNA standard
• The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to human genomic DNA standard, as well as step-by-step instructions
• Consistent performance For accurate quantitation of residual human DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample
• With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes
• *Due to the high degree of similarity between genomes, DNA from closely-related primate species such as monkey (Vero), chimpanzee, and orangutan are expected be detected by the resDNASEQ Human Quantitation Kit.