A21178
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A21178-Each-of-1 | In Stock | ₹ 84,533.28 |
A21178 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 84,533.28
GST (18%): ₹ 15,215.99
Total Price: ₹ 99,749.27
Cloning Method
Restriction Enzyme/MCS
Detection Method
Primer-probe
Format
Kit
Technique
CRISPR-Cas9
Product Type
Crispr Nuclease Vector With ofP Reporter Kit
Protein Tag
OFP fusion
Sufficient For
10 Reactions
No. of Reactions
10 Reactions
Starting Material
Oligos (DNA)
Content And Storage
• CRISPR OFP Nuclease Vector, linearized• 10X Oligonucleotide Annealing Buffer• DNase/RNase-free Water• 5X Ligation Buffer• T4 DNA Ligase• U6 Forward Sequencing Primer• Control, double stranded oligonucleotideStore the above at -5 to -30°C.• OneShot™ TOP10 Chemically Competent CellsStore at -80°C.
Form
Liquid
Reaction Speed
Fast
Product Line
GeneArt™
Promoter
U6, CMV
Reporter Gene
OFP
For Use With (Application)
Gene Expression, Cloning
Overhang
3' overhangs
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Description
- Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter
- GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter
- The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells
- The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease
- Competent cells included for added convenience and highest cloning efficiencies
- GeneArt™ CRISPR Nuclease Vector system offers: Easy-to-design genome engineering system Affordable, ready-to-use cloning vectors Enrichment for hard to transfect or difficult to modify cell For research use only
- Not for use in diagnostic procedures
- Certifications CoA Compliance CoA Recommended Storage Store in freezer (-5 to -30°C)