Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes

Description

• Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR
• Featuring an error rate 50-fold lower than that of Taq , and 6-fold lower than that of Pfu , Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity
• Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase
• Highlights Reaction set up at room temperature No non-specific amplification and primer degradation during reaction set up Zero-time reactivation due to unique hot start technology High fidelity (52X Taq) Fast PCR due to short extension times (15-30 s/kb) Robust reactions, minimal optimization needed Increased product yields with minimal enzyme amounts Direct loading on gels With Phusion Hot Start II High-Fidelity DNA Polymerases amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature
• The Affibody ligand also inhibits the 3'→5' exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up
• At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active
• Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures so it does not require a separate activation step in PCR protocols
• Phusion Green Hot Start II High-Fidelity PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps
• The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl 2
• The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel
• Applications High-fidelity PCR High throughput Amplification of difficult (GC-rich) templates Template generation for sequencing Multiplex PCR Long-range PCR Cloning Mutagenesis Microarray Using Phusion DNA Polymerases Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).