Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Description

• Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT
• The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain
• RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained
• RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C)
• Features of RevertAid H Minus Reverse Transcriptase include: • High yields of full-length first strand cDNA up to 13 kb • Optimum activity at 42–45°C • Active up to 55°C • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides) Applications • First strand cDNA synthesis for RT-PCR and real-time RT-PCR • Reverse transcription at elevated temperatures to reduce effects of secondary structure • Synthesis of cDNA for cloning and expression • Generation of labeled cDNA probes for microarrays • DNA labeling • Analysis of RNA by primer extension