Fractogel® EMD TMAE Hicap (M)

Description

• General description: Strong anion exchange Fractogel® resin featuring the tentacle technology with a trimethylammoniumethyl functional group for purification of acidic and neutral proteins and peptides from multiple sources, DNA and virus removal, plasmid purification, vaccine purification, and more
• Features and Benefits: Fractogel® EMD TMAE Hicap (M) enables users to:Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughputReduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitizationCompatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solutionDue to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).The strength of the binding depends on the following:the buffer systempH value of the buffer which determines the surface charge of the proteinthe concentration of the counter ionsthe charge density on the support (protein binding capacity)
• Packaging: 1.10316.0100: Fractogel® EMD TMAE Hicap (M) Resin 100ml1.10316.0010: Fractogel® EMD TMAE Hicap (M) Resin 10ml1.10316.0500: Fractogel® EMD TMAE Hicap (M) Resin 500ml1.10316.5000: Fractogel® EMD TMAE Hicap (M) Resin 5L
• Analysis Note: Appearance: Milky turbid suspension, free from impurities (foreign particles)Microscopic evaluation: Uniform spherical particles,no agglomerates,no finesExtractable matter (water): ≤ 0.03 %Cerium: ≤ 1 µg/gPressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 barParticle size (d10): 37 - 45 µmParticle size (d50): 48 - 60 µmParticle size (d90): 63 - 77 µmColony forming units (TAMC + TYMC): ≤ 100 CFU/mlEndotoxins: ≤ 1.00 EU/mlProtein binding capacity (bovine serum albumin): 160 - 200 mg/mlFunctional test (b:a): ≤ 0.25Functional test: Separation of conalbumin and human serum albumin
• Legal Information: FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany