Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Reagent Solution (OPA)

Description

• Thermo Scientific™ Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC
• Fluoraldehyde Reagent contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation
• The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC)
• Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations
• The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution
• Highlights: For pre- or post-column amino acid derivatization for fluorescent detection and quantitation Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives Provides an accurate measure of both composition and absolute protein-peptide content Ideal for work with recombinant proteins and synthetic peptides Can be used for fluorescent protein or peptide assay Pre-column derivatization mixtures can be injected into LC without any processing Reagent Properties: Formulation: 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10 Form: Clear, colorless to slightly yellow liquid Reactive toward: Primary amines (–NH2) Detection: Fluorescence (excitation/emission = 340nm/455nm) Application: Pre- or post-column derivatization for detection by HPLC