Thermo Scientific™ Pierce™ Saturated Ammonium Sulfate Solution

Description

• Thermo Scientific™ Pierce Saturated Ammonium Sulfate Solution is formulated and ready to use for simple immunoglobulin, antibody, or other protein fractionation applications based on selective precipitation, which is also called salting out
• The saturated ammonium sulfate solution (4.32M) enables removal of abundant proteins from serum, cell culture supernatant and ascites fluid
• Slowly adding the solution to a protein sample results in decreased protein solubility and eventual protein precipitation as the charged ammonium sulfate ions compete with protein for water interaction
• High molecular weight proteins, such as antibodies, are among the first to precipitate
• When used in combination with antibody purification methods such as affinity chromatography, ammonium sulfate precipitation is ideal for achieving highly purified antibody samples
• Highlights: Convenient – solution is ready to use; no preparation required Concentrates – recover antibody in 100mL from more than 1L of cell culture supernatant Improves purity – partial-purification of antibodies by ammonium sulfate precipitation greatly improves the final purity that can be obtained with affinity purification Extends column life – using pre-purified antibody samples instead of crude samples enables affinity columns to be used for many more cycles Compatible – ammonium sulfate is easily removed by dialysis or desalting columns, eliminating any obstacles to downstream methods Versatile – use for precipitating immunoglobulins or “salting out” fractions of other sample types