Invitrogen™ One Shot™ TOP10 Chemically CompetentE. coli

Description

• Description One Shot TOP10 Chemically Competent E
• coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 10 9 cfu/μg plasmid DNA
• These cells allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits
• TOP10 E
• coli cells are genetically similar to the DH10B strain and have been reported to be more resilient to stress conditions like osmotic shock and acidic pH stress
• One Shot TOP10 cells: • Maximize cloning efficiency in a single-tube format • Provide enhanced genomic DNA cloning capabilities TOP10 Chemically Competent E
• coli cells carry mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ) allowing the cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes
• Similar to other DH strains, TOP10 has the lac ZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal
• The inclusion of rec A1 and end A1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps
• One Shot TOP10 Chemically Competent E
• coli cells offer: • Transformation efficiencies of >1 x 10 9 cfu/μg • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones • end A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA • Expression from the lac promoter without IPTG Easy-to-use One Shot format TOP10 Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• The single-tube, single-use format accomodates allows all steps of the transformation protocol, up to plating, to take place in the same tube, helping save time and prevent contamination
• Genotype F – mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara-leu )7697 gal U gal K λ– rps L(Str R ) end A1 nup G Find the strain and format that fit your needs • We offer other DH strains in chemically competent and electrocompetent cell formats
• • The TOP10 strain is available in several MultiShot formats for high throughput applications
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