Thermo Scientific™ BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)

Description

• Thermo Scientific™ Pierce BS(PEG)n Crosslinkers include BS(PEG)5 and BS(PEG)9, which are bis-succinimide ester-activated PEG compounds for crosslinking between primary amines in proteins and other molecules
• BS(PEG)5 is a bis-succinimide ester-activated PEG compound used for crosslinking between primary amines (—NH2) in proteins and other molecules
• The N -hydroxysuccinimide ester (NHS) groups at either end of the PEG5 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds
• Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format
• The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial
• The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability
• Features of BS(PEG)5: Reactive groups: NHS ester (both ends) Reactive towards: amino groups (primary amines) NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds Water-soluble; compare to non-pegylated BS3 (also soluble) (Cat
• No
• 21580) and DSS (water-insoluble) (Cat
• No
• 21655) Membrane-impermeable, allowing for cell surface labeling Irreversibly crosslink proteins or peptides by flexible PEG spacer arms Polyethylene glycol spacer arms help maintain conjugate solubility Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity Ideal for small molecule or peptide conjugations Alternative Names for BS(PEG)n: Bis-NHS-dPEG NHS-PEG-NHS BS(PEO)5 Bis(NHS)PEO5 BS-PEO9 dPEG NHS ester Bis-succinimide ester PEG 400 Product References Crosslinker Application Guide -- search for recent literature references for this product Bioconjugate Techniques, Academic Press (Cat
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• 20036) Related Products BS(PEG)9 (PEGylated bis(sulfosuccinimidyl)suberate) (Cat
• No
• 21582) BS3 (bis(sulfosuccinimidyl)suberate) (Cat
• No
• 21580)