7180329
Thermo Scientific™ EZ-Link™ HPDP-Biotin, No-Weigh™ Format
Manufacturer: Fischer Scientific
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Solubility
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label Type
Biotin & Analogs
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Description
- Thermo Scientific™ EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods
- Highlights: Pyridyldithiol reacts with -SH groups to form a stable disulfide bond Absorbance of pyridine-2-thione leaving group at 343nm can be used to determine reaction progress Disulfide bond is cleavable using 50mM DTT (Part No
- 20291) or 100mM β-Mercaptoethanol (Part No
- 35600) Extended spacer arm prevents steric hindrance Must be dissolved in DMSO or DMF before use Biotinylation occurs between pH 6-9