Thermo Scientific™ EZ-Link™ TFP Ester-PEG4-DBCO

Description

• An amine reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications The crosslinker can be used to label a variety of amine containing molecules such as proteins, biomolecules, or small molecules Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately) The coupling or crosslinking procedure is typically done in two steps First, a protein or amine containing molecule is labeled with a DBCO group and excess crosslinker is removed Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst Ragent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures TFP esters are more hydrolytically stable than NHS esters