Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit - Mini-Spin Columns

Description

• This innovative antibody-labeling system uses nickel-chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions
• Once held in place on the resin, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines
• Excess labeling reagent and byproducts are then washed away before recovering the labeled and purified antibody from the resin using a mild imidazole solution
• No gel filtration or dialysis is needed
• Four kits are available for small (0.1-1mg) or large (1-10mg) antibody samples using either amine-directed (NHS ester) or sulfhydryl-directed (maleimide) labeling reagents
• Highlights: Fast labeling and purification - the entire procedure takes only one hour (two hours for sulfhydryl labeling kits) Easy removal of spent and excess labeling reagent - simply wash away the reaction byproducts - no need for dialysis or gel filtration No dilution effects - solid-phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling Optimized protocols - specific protocols for antibody ensure appropriate level of labeling (2-5 biotins per antibody molecule), minimizing possibility of inactivation caused by overlabeling Sufficient reagents for eight biotin-labeling experiments - Thermo Scientific No-Weigh Packaging ensures that the biotin reagent is fully active for eight separate experiments