Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)

Description

• Thermo Scientific™ Pierce PEG4-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 4-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond
• SPDP reagents have an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a suflhydryl-reactive 2-pyridyldithio group at the opposite end
• PEG4-SPDP has a 4-unit polyethylene glycol spacer arm, which confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers
• Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT)
• Crosslinking experiments with SPDP reagents are not limited to those involving proteins
• Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using an SPDP reagent
• Highlights: Efficient amine conjugation – NHS ester provides effective conjugation to lysines, N-termini of peptides and other primary amines Reversible sulfhydryl conjugation – pyridyldithio group enables disulfide linkage with reduced cysteines and other sulfhydryl groups, providing a stable but cleavable bond Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and often enhance the solubility of attached molecules Spacer arm – connects primary amines and sulfhydryl targets at distances up to 25.7 angstroms Specifications: Chemical name (simplified): 2-pyridyldithiol-tetraoxatetradecane-N-hydroxysuccinimide Chemical formula: C23H33N3O9S2 Molecular weight: 559.65 Spacer arm: 25.7 angstroms Form: straw to amber colored oil Solubility: dissolve 2mg in 179μL DMSO or DMF to make 20mM stock for each use