Thermo Scientific™ iodoTMTsixplex™ Isobaric Label Reagents and Kits

Description

• Thermo Scientific iodoTMT Label reagents enable multiplexed identification and quantitation of cysteine containing peptides in different samples using tandem mass spectrometry
• IodoTMT reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (–SH) groups
• iodoTMTzero Label Reagent allows testing and optimization of sample preparation, labeling, fractionation, and MS fragmentation for peptide identification and reporter detection without using the more costly isotope-labeled compounds
• Iodoacetyl tandem mass tag reagents enable selective labeling of cysteine-containing peptides for multiplexed sample quantitation using LC-MS/MS analysis
• Similar to iodoacetamide, iodoTMT reagents react specifically with reduced cysteines (Cys) in peptides and proteins and enable quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation and the oxidation of disulfide bonds
• IodoTMT reagents irreversibly label free sulfhydryl groups on cysteine residues, in contrast to previous reversible cysteine-reactive TMT (cysTMT) reagents
• To selectively analyze the cysteine-labeled peptides, the sample may be enriched using Immobilized Anti-TMT Antibody Resin (Cat
• No
• 90076) and TMT Elution Buffer (Cat
• No
• 90104)
• This approach of selective thiol-labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT method) but allows for affinity enrichment with a non-biological tag and higher multiplex quantitation
• Features of iodoTMT reagents • Specific —only reacts with sulfhydryl groups (reduced, unmodified cysteine residues) • Irreversible —labeled proteins and peptides are not susceptible to reducing agents • Flexible —options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation • Versatile —can be used to study cysteine modifications (e.g., di-sulfide bridges, S-nitrosylation)