Thermo Scientific™ iodoTMTsixplex™ Isobaric Mass Tagging Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Pack of 1 | PI90103-Pack-of-1 | In Stock | ₹ 2,98,604.40 |
PI90103 - Pack of 1
In Stock
Quantity
1
Base Price: ₹ 2,98,604.40
GST (18%): ₹ 53,748.792
Total Price: ₹ 3,52,353.192
Description
iodoTMTsixplex™ Isobaric Mass Tagging Kit
Product Line
iodoTMTsixplex™
Label or Dye
Tandem Mass Tag™ (TMT) Labels
Final Product Type
Protein
Workflow Step
Protein Labeling
For Use With (Equipment)
Mass Spectrometer
Content And Storage
• iodoTMT6-126 Label Reagent, 5 x 0.2 mg• iodoTMT6-127 Label Reagent, 5 x 0.2 mg• iodoTMT6-128 Label Reagent, 5 x 0.2 mg• iodoTMT6-129 Label Reagent, 5 x 0.2 mg• iodoTMT6-130 Label Reagent, 5 x 0.2 mg• iodoTMT6-131 Label Reagent, 5 x 0.2 mg• HES Buffer, 15 mL• Bond-Breaker TCEP Solution, 0.5 mL• DTT, No-Weigh Format, 8 x 7.7 mg• Trypsin, 5 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin (bovine), 2.5 mgUpon receipt store all products at –20°C
Starting Material
Cell Lysate
Detection Method
Mass Spectrometry
Quantity
30 reactions
Related Products
Description
- Thermo Scientific iodoTMTsixplex Isobaric Mass Tag Labeling Kit contains iodoTMT™ reagents and associated materials for the irreversible labeling of sulfhydryl groups
- Iodoacetyl Tandem Mass Tag™ Reagents enable selective labeling of cysteine-containing peptides for multiplexed sample quantitation using LC-MS/MS analysis
- The iodoTMT Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups
- Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins and enable quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation and the oxidation of disulfide bonds
- The Thermo Scientific iodoTMTzero Label Reagent allows testing and optimization of sample preparation, labeling, fractionation and MS fragmentation for peptide identification and reporter detection without using the more costly isotope-labeled compounds
- Features of the iodoTMT Reagents: • Specific —only reacts with sulfhydryl groups (reduced, unmodified cysteine residues) • Irreversible —labeled proteins and peptides are not susceptible to reducing agents • Flexible —options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation • Versatile —can be used to study cysteine modifications (e.g
- di-sulfide bridges, S-nitrosylation) To selectively analyze the cysteine-labeled peptides, the sample may be enriched using the Thermo Scientific Immobilized Anti-TMT Antibody Resin (Cat
- No
- 90076) and TMT Elution Buffer (Cat
- No
- 90104)
- This approach of selective thiol-labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT™ Method) but allows for affinity enrichment with a non-biological tag and higher multiplex quantitation.