Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Description

• Thermo Scientific™ Pierce DTT (Dithiothreitol) is offered as 7.7 mg x 48-tube microplates for convenient preparation of 100 μL of 0.5 M stocks for addition to protein samples for effective reduction of disulfide bonds
• Thermo Scientific Pierce DTT (Dithiothreitol), No-Weigh format, is offered as 7.7 mg x 48-tube microplates for convenient preparation of 100 μL of 0.5 M single-use stocks for addition to protein samples for effective reduction of disulfide bonds
• DTT is a popular reducing agent for many protein research applications, including preparing proteins for reducing polyacrylamide electrophoresis (SDS-PAGE) and reducing disulfide bonds for labeling sulfhydryl groups in conjugation reactions
• At low concentrations, DTT is used to stabilize proteins and enzymes with free sulfhydryl groups to keep monothiols in a reduced state
• DTT reduction is also used to prepare proteins for mass spectrometry
• Properties of DTT: • Synonyms —1,4-dithiothreitol; DTT; Cleland’s Reagent; 1,4-dimercapto-2,3-butanediol • Molecular weight (MW) —154.24 • Molecular formula —C 4 H 10 O 2 S 2 • CAS number —3483-12-3 DTT is not stable in solution and should be prepared fresh for each use
• The No-Weigh format consists of a 48-microtube tray containing dry, stable, single-use aliquots of the DTT chemical to eliminate the need to weigh small amounts of reagent and prevent waste of unused DTT
• Just puncture the seal of one microtube, add 100 μL of water or buffer, and mix to dissolve
• In just a few seconds you will have a solution of 500 mM DTT, ready for a variety of laboratory methods
• Dilute as necessary or add the appropriate volume of the 500 mM stock solution to achieve the amount of DTT reducing agent required for your specific application
• Features of the No-Weigh format: • Saves time —just add 100 μL water or buffer to make 500 mM DTT solution in seconds • Eliminates waste —makes 100 μL at a time, just the right amount for most applications • Always fresh —ensures full reducing strength for every use; no need to store stock solutions With the convenient No-Weigh format, a 500 mM DTT solution can be made in less than 30 seconds
• The unique packaging ensures that the DTT reducing agent is at full strength and able to protect proteins from oxidative damage or reduce any disulfides prior to electrophoresis.