Invitrogen™ BACE1 (β-Secretase) FRET Assay Kit, red
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-257-6137-Each-of-1 | In Stock | ₹ 1,20,810.72 |
50-257-6137 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,20,810.72
GST (18%): ₹ 21,745.93
Total Price: ₹ 1,42,556.65
Content And Storage
Components of the BACE1 FRET Assay Kit are listed in Table 1. Sufficient reagent is supplied for 400 high-throughput kinetic or endpoint assays. The BACE1 Enzyme is also available separately. Store BACE1 Enzyme at -80°C. Store BACE1 Substrate and BACE1 Product Standard at -20°C. Avoid subjecting these components to repeated freeze-thaw cycles. The BACE1 Assay Buffer and BACE1 Stop Solution can be thawed upon receipt and stored at room temperature.
Product Type
FRET Assay Kit
Substrate Type
Beta-Secretase Substrate
Quantity
400 x 40 μL Assays
Target
Beta Secretase (BACE1)
Assay
β-Secretase Assay
Substrate Properties
Peptide-Based Substrate
For Use With (Application)
FRET
Shipping Condition
Dry Ice
Related Products
Description
- BACE1 (β-secretase) is a key enzyme involved in the production of Amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimers disease (AD)
- In some cases, early onset familial AD can be attributed to a Swedish mutation in the amyloid precursor protein (APP), dramatically enhancing the cleavage of this protein by BACE1
- This and other genetic and pathological evidence has led to therapeutic approaches focusing on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase
- The BACE1 fluorescence resonance energy transfer (FRET) Assay Kit provides a sensitive and efficient method for screening potential BACE1 inhibitors
- This kit uses purified baculovirus-expressed BACE1 and a new red FRET peptide substrate based on the Swedish mutant
- The BACE1 FRET Assay Kit offers: Red emission to reduce compound interference Excitation at 545 nm and read emission at 585 nm An easy-to-use, rapid, homogeneous assay that is performed in solution Versatile formatting for high-throughput screening Stable results—measurements can be made for up to 24 hours The principle of the BACE1 FRET assay is as follows: The peptide substrate is synthesized with two fluorophores, a fluorescent donor (a rhodamine (Rh) derivative), and a proprietary quenching acceptor
- The distance between these two groups has been selected so that upon light excitation, the donor (D) fluorescence energy is significantly quenched by the acceptor (A) through a quantum mechanical phenomenon known as resonance energy transfer (Figure 1)
- Upon cleavage by the protease, the fluorophore is separated from the quenching group, restoring the full fluorescence yield of the donor
- Thus, a weakly fluorescent peptide substrate becomes highly fluorescent upon enzymatic cleavage; the increase in fluorescence is linearly related to the rate of proteolysis
- Source of BACE1 Enzyme: A cDNA sequence encoding amino acids 1-460 of human BACE1, corresponding to the ectodomain, was expressed in recombinant, baculovirus-infected insect cells
- Purified BACE1 exists as the proBACE1 form having an apparent molecular weight of 55 kDa and an N-terminal sequence of TQHGIRLPLR.