K145501

Invitrogen™ β-Gal Assay Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 K145501-Each-of-1 In Stock ₹ 39,015.36

K145501 - Each of 1

₹ 39,015.36

In Stock

Quantity

1

Base Price: ₹ 39,015.36

GST (18%): ₹ 7,022.765

Total Price: ₹ 46,038.125

Content And Storage

The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.

Detection Method

Colorimetric

Substrate

ONPG

Target

Beta-Galactosidase

Assay

β-Gal (lacZ) Assay

Product Type

Beta-Gal Assay Kit

Quantity

80 mL

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Description

  • lacZ is bacterial gene often used as reporter construct in eukaryotic transfection experiments Gene product of lac Z, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG) Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer 10.7 gene expression Colorimetric detection Beta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays