Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16183-Each-of-1 | In Stock | ₹ 14,699.21 |
PI16183 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,699.21
GST (18%): ₹ 2,645.858
Total Price: ₹ 17,345.068
Type
Assay Kit
Sufficient For
100 Dual Luciferase Assays
Content And Storage
Sufficient For: 100 dual-luciferase assays in microplate wells• Cypridina-Firefly Dual Assay Buffer, 5 mL (store at 4°C)• 100X Vargulin, 50 μL (store at -20°C)• D-Luciferin, 3 mg (store at 4°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Upon receipt, store kit at -20°C, or store individual components as indicated above.
Compatible Cells
Mammalian Cells
For Use With (Equipment)
Luminometer (Microplate)
Label Type
Enzyme Labeled
Technique
Enhanced Chemiluminescence
Product Line
Pierce™
Target
Luciferase, Cypridina Luciferase
Quantity
100 Reaction Kit
Assay
Reporter Enzyme, Luciferase Reporter Assay
Detection Method
Bioluminescence
Format
384-well plate, 96-well plate
Substrate Properties
Chemical Substrate
Substrate Type
Luciferase Substrate
Substrate
Vargulin (Cypridina Luciferin), D-Luciferin
Related Products
Description
- The Thermo Scientific™ Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates
- The Pierce Cypridina -Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity
- Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control
- This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching
- The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics
- The resulting luminescent signals are spectrally resolvable using filters
- In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors
- Highlights: Simultaneous – concurrent, filter-based, wavelength-separated detection of two luciferase activities Sensitive – measure bright blue Cypridina and red firefly luciferase activities in the same sample Fast – no quenching step required, unlike in traditional sequential dual assays Multiplex – capable of quantitating two cellular activities in the same sample(s) Includes: Cell lysis buffer, reaction buffer, and substrates Vargulin and D-luciferin Requires: Cypridina and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Cypridina Luc and 615 to 675nm for Red Firefly Luc
- Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time
- Recommended for: Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g
- off-target effects)