7180360
Invitrogen™ Avidin Protein, HRP
Manufacturer: Fischer Scientific
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Target Molecule
Biotin
Specificity
Biotin
Form
Solid
Label or Dye
HRP (Horseradish Peroxidase)
Content And Storage
Store at -20°C. Shipped at ambient temperature.
Enzyme
HRP (Horseradish Peroxidase)
For Use With (Application)
Western Blotting, ELISA, Immunohistochemistry
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Description
- Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods
- Pure avidin is a key component in certain immunohistochemistry (IHC) methods
- Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates
- Highlights: Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10 Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin Affordable – significantly less expensive than recombinant streptavidin ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC) Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays