Cytiva™ HiScreen™ Column with MabSelect™ VL Protein L Resin

Description

• As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants
• As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain
• Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1
• Redesigned protein L ligand MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to Cytiva™ Capto™ L chromatography resin
• This higher capacity provides productivity advantages in research and biomanufacturing
• A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability High dynamic binding capacity for efficient capture of target molecules Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents Provides good resolution for product-related impurities in the capture of bispecific antibodies Convenient for method optimization and parameter screening enabled by the 10 cm bed height for efficient chromatography process development