45-002-072
Cytiva HiPrep™ IMAC FF 16/10 Column
Manufacturer: Cytiva
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 45-002-072-Each-of-1 | In Stock | ₹ 62,287.68 |
45-002-072 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 62,287.68
GST (18%): ₹ 11,211.782
Total Price: ₹ 73,499.462
Buffer
0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol
Bed Volume
20mL
Diameter (Metric) Inner
16mm
Height (English)
3.93 in.
Flow Rate
<10mL/min.
Max. Pressure
1.5 bar
Particle Size
45 to 165μm
Content And Storage
4° to 30°C
For Use With (Application)
IMAC and subsequent purification of Polyhistidine tagged Proteins
Product Type
HiTrap IMAC Sepharose 6 FF column
Diameter (English) Inner
0.62 in.
Quantity
1 x 20mL
Height (Metric)
100mm
Material
Polypropylene
pH Range
3 to 12
Support Type
6% Cross-linked Agarose
Column Type
Ion Exchange Column
Related Products
Description
- IMAC Sepharose™ 6 Fast Flow is prepacked in 20mL HiPrep columns for greater scale up
- The medium consists of 90μm beads of highly crosslinked agarose, to which a chelating group has been covalently coupled
- This group may be charged with suitable metal ions, specific for your target protein.The bead size enables high flow properties; low metal ion leakage retains activity of the purified protein
- Specifications: IMAC Sepharose 6 Fast Flow Matrix: highly crosslinked agarose, 6% Average particle size: 90μm Binding capacity (protein/ion dependent): approx
- 40mg histidine-tagged protein/mL medium when charged with Ni 2+ Compatibility: stable in all commonly used buffers Chemical stability (uncharged medium) one week, 40°C: 0.01 M HCl, 0.1 M NaOH; 12 hr.: 1M NaOH, 70% acetic acid; 1 hr
- tested 2% SDS; 30 min
- tested 30% 2-propanol Stability (pH, w/ uncharged medium w/ metal ion): 3 to 12 (long-term), 2 to 14 (short-term) Storage/Temperature: 20% ethanol; 4° to 30°C HiPrep Prepacked Columns Volume: 20mL Dimensions: 1.6 x 10cm Max
- pressure during operation: 1.5bar (0.15MPa, 22psi) Hardware pressure limit: 5bar (0.5MPa, 73psi) Recommend flowrate (H 2 O at room temp.): 2 to 10mL/min.