Thermo Scientific™ Pierce™ Glutathione Superflow Agarose

Description

• Perform high-purity, large-scale purification of GST-tagged proteins with Thermo Scientific™ Pierce Glutathione Superflow Agarose, which consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose
• Additional Information and Online Ordering Pierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications
• The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures
• The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process
• Highlights: High dynamic binding capacity – binds 10mg of pure GST per mL of resin at a linear flow rate of 150cm/hour; can bind greater than 20mg/mL at 30cm/hour flow rate High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour Compatible – maintains function after exposure to a wide variety of chemicals and pH values Cost effective – resin is stable through multiple cycles of cleaning and reuse Recommended for: Medium- to large-scale FPLC purification of GST-tagged proteins