Thermo Scientific™ CarboxyLink™ Immobilization Kit, 2 mL

Description

• Thermo Scientific™ CarboxyLink Coupling Resins and Kits provide for the covalent immobilization of peptides or other carboxyl-containing (-COOH) molecules to a porous, beaded resin for use in affinity purification procedures
• These activated resins enable immobilization of carboxylate ligands for use in affinity protocols
• CarboxyLink Resin is crosslinked beaded agarose (or acrylamide-based Thermo Scientific UltraLink™ Biosupport) that has been activated with diaminodipropylamine (DADPA) to contain long spacer arms, each of which have a primary amine at the end
• When incubated with the resin and the carbodiimide crosslinker EDC (included in the CarboxyLink Immobilization Kit), carboxyl-containing molecules become permanently attached to the support by stable amide bonds
• Once a molecule is coupled, the prepared affinity column can be used multiple times in typical protein affinity purification procedures
• CarboxyLink Coupling Resins also can be used to immobilize other kinds of molecules using alternative amine-reactive crosslinking chemistries
• Highlights: CarboxyLink Coupling Resins – beaded agarose or acrylamide support activated with diaminodipropylamine (DADPA) for amine-to-carboxyl conjugation using EDC crosslinker Efficient peptide immobilization – easily couple 1 to 2mg of peptide per milliliter of settled resin Stable linkage – immobilization results in covalent attachment of carboxyl groups by amide bonds, allowing for multiple rounds of affinity purification with one batch of prepared resin Flexible and gentle coupling conditions – immobilization reaction completed in simple MES or other non-amine and non-carboxyl, near-neutral buffer, with or without organic solvent Ideal for unmodified peptides – immobilizes peptides with high capacity and various orientations without steric hindrance, allowing for effective use in affinity purification of specific antibodies Convenient kits and product sizes – choose 5-column kits with complete sets of buffers, crosslinker and versatile spin/drip columns containing either type of resin (agarose or polyacrylamide) or choose stand-alone resin for other uses CarboxyLink Coupling Resin (Part No
• 20266, 44899): Ligand: diaminodipropylamine (DADPA) Support: crosslinked 4% beaded agarose Spacer arm: 9 atoms Loading: activated with at least 16μmol amine per milliliter of settled resin Format: 50% resin slurry in 0.05% sodium azide UltraLink DADPA Resin (Part No
• 53154): Ligand: diaminodipropylamine (DADPA) Support: Thermo Scientific UltraLink Biosupport Spacer arm: 14 atoms Loading: activated with at least 40μmol amine per milliliter of settled resin Format: 50% resin slurry in 0.02% sodium azide Includes: CarboxyLink Resin Columns (DADPA agarose or UltraLink Support), EDC crosslinker, coupling buffer (MES-buffered saline), wash solution, and column accessories