Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Description

• Preserve phosphorylation of proteins in cells and tissues with Thermo Scientific™ Halt Phosphatase Inhibitor Cocktail
• Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases, as well as protein tyrosine phosphatases (PTPs) during cell lysis The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell including signal transduction, cell division, and apoptosis To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis Additional Features Devoid of protease inhibitors – To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail (78420) Dialyzable – Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components Supplied as a 100X concentrate – effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X Compatibility – compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases Compatible with popular protein assays – works with standard protein assays, including BCA and coomassie (Bradford) assays Convenient storage – refrigerator storage (2 to 8°C) saves valuable space in the freezer and allows for immediate use