Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis

Description

• Thermo Scientific™ Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers
• Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens
• The enzyme is produced and purified from E
• coli and consists of two identical 30-kDa subunits with two critical disulfide bonds
• This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH
• This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases
• Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250 U/μL
• Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™
• Highlights: Broad spectrum – degrades all forms of DNA and RNA Highest-quality enzyme – nuclease is at least 99% pure, as tested by SDS-PAGE Robust activity – 100-fold greater specific activity than DNase I Versatile – can be used with a wide variety of cell lysis reagents Recommended for: Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing Storage Requirements Store in a cool, dry, well-ventilated area, protected from direct sunlight