7184499
Thermo Scientific™ L-Proline for SILAC
Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.
Manufacturer: Fischer Scientific
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Description
L-Proline
Product Type
Amino Acids
Label or Dye
Mass Isotope of Amino Acid
Starting Material
Cells
Detection Method
Mass Spectrometry
For Use With (Equipment)
Mass Spectrometer
Content And Storage
Store at room temperature.
Shipping Condition
Room Temperature
Final Product Type
Protein
Workflow Step
Protein Labeling
Related Products
Description
- Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits
- SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples
- This approach entails the in vivo metabolic incorporation of “heavy“ 13 C- or 15 N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins
- Highlights: Efficient – 100% label incorporation into proteins of living cells Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment High-quality supplements – heavy amino acids with >99% isotope purity