Thermo Scientific™ Pierce™ Streptavidin, Hydrazide-Activated

Description

• Thermo Scientific™ Pierce Streptavidin and Conjugates include purified recombinant streptavidin biotin-binding protein, plus standard SA-HRP, SA-AP and fluorescent conjugates for biotin-based assays
• These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging
• Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water
• Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer
• Highlights: Recombinant streptavidin – high-quality protein, 53kDa, pI equals 6.8 to 7.5 Pure protein and enzyme conjugates – choose pure, unconjugated protein for biotin blocking and controls; enzyme conjugates for substrate-based detection Fluorophore conjugates – standard fluorescent forms (fluorescein, rhodamine, etc.) for traditional immunofluorescence techniques Activated preparations – choose a maleimide-activated or hydrazide-activated form to prepare a custom streptavidin conjugate with sulfhydryl- or carbohydrate-containing molecule