SAE0092

Mutanolysin from Streptomyces globisporus ATCC 21553

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)

Manufacturer: Sigma Aldrich

CAS Number: 55466-22-3

Synonym(S): Mutanolysin

Select a Size

Pack Size SKU Availability Price
10 KU SAE0092-10-KU In Stock ₹ 21,978.00

SAE0092 - 10 KU

₹ 21,978.00

In Stock

Quantity

1

Base Price: ₹ 21,978.00

GST (18%): ₹ 3,956.04

Total Price: ₹ 25,934.04

biological source

Streptomyces sp. (Streptomyces globisporus ATCC 21553)

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa ~18.5 kDa

feature

DNA free

concentration

≥40% protein

technique(s)

nucleic acid detection: suitable

suitability

suitable for cell lysis

application(s)

life science and biopharma

shipped in

wet ice

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Description

  • Application: Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
  • Biochem/physiol Actions: Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
  • Features and Benefits: Suitable for difficult-to-lyse samples. Microbial DNA free, minimizing DNA contamination from reagents in workflow. Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
  • Unit Definition: One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
  • Physical form: Lyophilized powder containing Ficoll® and sodium succinate buffer salts
  • Legal Information: Ficoll is a registered trademark of Cytiva

SAFETY INFORMATION

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable