Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli

Description

• General description: Recombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
• Warning: Toxicity: Standard Handling (A)
• Unit Definition: One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol porcine fibrinogen per min at 37°C, pH 5.5.
• Other Notes: Tarentino, A.L., et al. 1995. Glycobiology 5, 599.Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
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