Description

• Application: β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.
• Biochem/physiol Actions: βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.
• Unit Definition: 1 U corresponds to the amount of enzyme which liberates 1 μmol glucose per minute at pH 5.0 and 37 °C (salicin as substrate)
• Other Notes: Role of β-glucosidase in cellulose hydrolysis: Review[1]; Enzymic hydrolysis of cellulose for fuel ethanol production by simultaneous saccharification and fermentation[2]; Stereospecific attachment of carbohydrates to amino acid derivatives[3][4]; Enzyme-catalyzed synthesis of alkyl β-D-glucosides in organic media[5]