C7762
α-Chymotrypsin from bovine pancreas
Type I-S, essentially salt-free, lyophilized powder
Manufacturer: Sigma Aldrich
CAS Number: 9004-07-3
Synonym(S): α-chymotrypsin A and B, alpha-chymotrypsin
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 MG | C7762-5-MG | In Stock | ₹ 4,129.20 |
| 25 MG | C7762-25-MG | In Stock | ₹ 8,935.50 |
| 100 MG | C7762-100-MG | In Stock | ₹ 28,360.50 |
C7762 - 5 MG
In Stock
Quantity
1
Base Price: ₹ 4,129.20
GST (18%): ₹ 743.256
Total Price: ₹ 4,872.456
type
Type I-S
Quality Level
200
form
essentially salt-free, lyophilized powder
specific activity
≥40 units/mg protein
mol wt
25 kDa
purified by
3× crystallization
solubility
1 mM HCl: soluble 2.0 mg/mL, clear
UniProt accession no.
P00767
storage temp.
−20°C
Gene Information
cow ... CTRB1(618826)
Other Options
Related Products
Description
- Application: α-Chymotrypsin from bovine pancreas has been used in a study to investigate protein extraction by Winsor-III microemulsion systems. α-Chymotrypsin from bovine pancreas has also been used in a study to investigate a new specific fullerene-based fluorescent probe for trypsin.
- Biochem/physiol Actions: α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, as well as 10 mM of Cu2+ and Hg2+.
- Unit Definition: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
- Preparation Note: Prepared free of autolysis products and low molecular weight contaminants.
- Analysis Note: Minimum 85% protein
- Other Notes: View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves