C9268
Carboxypeptidase A from bovine pancreas
(Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution
Manufacturer: Sigma Aldrich
CAS Number: 11075-17-5
Synonym(S): Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 UNITS | C9268-500-UNITS | In Stock | ₹ 16,694.40 |
| 5000 UNITS | C9268-5000-UNITS | In Stock | ₹ 44,832.90 |
C9268 - 500 UNITS
In Stock
Quantity
1
Base Price: ₹ 16,694.40
GST (18%): ₹ 3,004.992
Total Price: ₹ 19,699.392
grade
Proteomics Grade
Quality Level
300
form
ready-to-use solution
quality
(Type II-PMSF treated)
specific activity
≥50 units/mg protein
mol wt
~35 kDa
purified by
2× crystallization
impurities
≤0.05 BTEE units/mg protein chymotrypsin≤10 BAEE units/mg protein trypsin
storage temp.
2-8°C
Other Options
Related Products
Description
- Application: Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.
- Biochem/physiol Actions: Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.[3]
- Unit Definition: One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.
- Preparation Note: Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.
- Analysis Note: Protein determined by E1%/278