Description

• General description: A product of Novozymes Corp.
• Application: Dextranase from Chaetomium erraticum has been used:in microcarrier dissolution to produce multicellular 3D spheroids for drug discovery[2]in in vitro digestion of extracellular polysaccharide (EPS) from the bacterial isolates[3]in a study to investigate the immobilization of dextranase[4]to investigate the optimization of process conditions for enzymatic modification of alternan
• Biochem/physiol Actions: Dextranase is an endodextranase that hydrolyzes α-(1,6)-glycosidic linkages in dextran. It has many industrial and clinical applications such as food processing, sugar modification, and medicine preparation.[1] Dextranase is widely used to produce alternated sugar beet and isomaltooligosaccharides (IMOs), a prebiotic functional food.[5][1] The low molecular weight dextran obtained from dextranase serves as a synthetic blood volume expander.[1] Dextranase can be coupled to antibodies for the detection of tumor cells.[5] It prevents the occurrence of dental plaques by depolymerizing microbial dextran deposits in teeth. Hence, it is used in dental care products.[1]
• Preparation Note: A fungal dextranase produced by submerged fermentation of Chaetomium erraticum.
• Analysis Note: Stable in the pH range of 3-7 and at temperatures up to approx. 70 °C. For most applications, the preferred conditions are pH 5-6 and a temperature of 50-60 °C.
• Other Notes: View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer